The authors would like to declare the following patent applications associated with this research: “Pet food composition and method of making pet food composition comprising enhanced levels of resistant starch” (WO2019112562A1). The funder was given the opportunity to read the manuscript prior to publication, but played no further role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have read the journal’s policy and have the following competing interests: MIJ and CW are current employees of Hill’s Pet Nutrition, and DEJ is a former employee of Hill's Pet Nutrition, which provided funding for this study. The specific roles of these authors are articulated in the ‘author contributions’ section. All other data are available in the paper and its Supporting Information files.įunding: Hill's Pet Nutrition provided funding for this study in the form of salaries for MIJ, CW, and DEJ. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Sequences were deposited in the NCBI Sequence Read Archive under accession number PRJNA63518. Received: NovemAccepted: OctoPublished: November 3, 2020Ĭopyright: © 2020 Jackson et al. Loor, University of Illinois, UNITED STATES These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.Ĭitation: Jackson MI, Waldy C, Jewell DE (2020) Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat. Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks. At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types many involved taxa with known saccharolytic or proteolytic proclivities. Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors. RS consumption altered 47% of the fecal metabolome RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased. Fecal IgA concentrations were significantly higher at week 6 with HRS food. In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats. Few differences were seen in proximate analyses of the foods stool firmness scores did not differ. Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome fecal immunoglobulin A (IgA) was analyzed at week 6. Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels, respectively). The degree to which cats manifest beneficial changes in response to RS intake was examined.
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